Melatih Keterampilan Literasi Sains Mahasiswa Melalui Uji Kualitas Mikrobiologi Menggunakan Metode Perhitungan Angka Total
Keywords:
Scientific Literation, Standar plate Count, MicroorganismsAbstract
Food was consumed in everyday life. Various and derivation types of processed products that we consume are obtained from industrialized products that are oriented towards the processing of the food industry. Processed food products in the form of fish that go through various kinds of management can be contaminated with thermal temperatures and bacteriophages which are parasitic and have a negative impact on health. The research carried out aims to test the quality of microbes contained in fish that have been preserved using various types of preservatives. This research procedure uses the Standard Plate Count (ALT) technique which can provide a representative test for the types of microbes found in cured tilapia fish at certain time intervals. The results of the research data were analyzed using the ANOVA test which consisted of 5 repetitions, this was aimed at determining the average distribution of each repetition of the research procedures carried out showing coherent results. The results showed that the ALT sampling technique was effective in determining colonies of bacteriophages and other micro-organisms (pathogenic microorganisms) present in preserved tilapia fish that could differentiate into viruses that are harmful to public health.
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Bartold, Katarzyna, Agnieszka Pietrzyk-Le, Wojciech Lisowski, Karolina Golebiewska, Alexandra Siklitskaya, Pawel Borowicz, Shuai Shao, Francis D’Souza, and Wlodzimierz Kutner. 2019. “Promoting Bioanalytical Concepts in Genetics: A TATA Box Molecularly Imprinted Polymer as a Small Isolated Fragment of the DNA Damage Repairing System.” Materials Science and Engineering C 100(February):1–10. doi: 10.1016/j.msec.2019.02.038.
Choi, Inyoung, Da Som Yoo, Yoonjee Chang, So Yeon Kim, and Jaejoon Han. 2021. “Polycaprolactone Film Functionalized with Bacteriophage T4 Promotes Antibacterial Activity of Food Packaging toward Escherichia Coli.” Food Chemistry 346(November 2020):128883. doi: 10.1016/j.foodchem.2020.128883.
Corebima, Aloysius Duran. 2016. “Pembelajaran Biologi Di Indonesia Bukan Untuk Hidup.” Seminar Nasional XIII Biologi, Sains, Lingkungan, Dan Pembelajarannya Di Pendidikan Biologi FKIP UNS 13(1):8–22.
Corella, Dolores, and Jose M. Ordovas. 2017. “Basic Concepts in Molecular Biology Related to Genetics and Epigenetics.” Revista Española de Cardiología (English Edition) 70(9):744–53. doi: 10.1016/j.rec.2017.05.011.
Costa, Maria J., Lorenzo M. Pastrana, José A. Teixeira, Sanna M. Sillankorva, and Miguel A. Cerqueira. 2021. “Characterization of PHBV Films Loaded with FO1 Bacteriophage Using Polyvinyl Alcohol-Based Nanofibers and Coatings: A Comparative Study.” Innovative Food Science and Emerging Technologies 69(November 2020). doi: 10.1016/j.ifset.2021.102646.
Gao, Jiaxi, Xiaoyu Hua, Ruo Yuan, Qiong Li, and Wenju Xu. 2021. “Amplified Electrochemical Biosensing Based on Bienzymatic Cascade Catalysis Confined in a Functional DNA Structure.” Talanta 234(July):122643. doi: 10.1016/j.talanta.2021.122643.
Garenne, David, Steven Bowden, and Vincent Noireaux. 2021. “Cell-Free Expression and Synthesis of Viruses and Bacteriophages: Applications to Medicine and Nanotechnology.” Current Opinion in Systems Biology 28:100373. doi: 10.1016/j.coisb.2021.100373.
Gíria, Marta, Luís Santos, João Louro, and Helena Rebelo de Andrade. 2016. “Reverse Genetics Vaccine Seeds for Influenza: Proof of Concept in the Source of PB1 as a Determinant Factor in Virus Growth and Antigen Yield.” Virology 496:21–27. doi: 10.1016/j.virol.2016.05.015.
Hambarde, Shashank, Chi Lin Tsai, Raj K. Pandita, Albino Bacolla, Anirban Maitra, Vijay Charaka, Clayton R. Hunt, Rakesh Kumar, Oliver Limbo, Remy Le Meur, Walter J. Chazin, Susan E. Tsutakawa, Paul Russell, Katharina Schlacher, Tej K. Pandita, and John A. Tainer. 2021. “EXO5-DNA Structure and BLM Interactions Direct DNA Resection Critical for ATR-Dependent Replication Restart.” Molecular Cell 81(14):2989-3006.e9. doi: 10.1016/j.molcel.2021.05.027.
Holmes, Tarquin. 2017. “The Wild Type as Concept and in Experimental Practice: A History of Its Role in Classical Genetics and Evolutionary Theory.” Studies in History and Philosophy of Science Part C :Studies in History and Philosophy of Biological and Biomedical Sciences 63:15–27. doi: 10.1016/j.shpsc.2017.03.006.
Hu, Yuqiang, Zhen Zhang, Weicong Ye, Wei Zhang, Minghao Hu, Wenqian Yuan, Hongbo Wang, Xianjin Xiao, and Tongbo Wu. 2021. “A DNA Structure-Mediated Fluorescent Biosensor for Apurinic/Apyrimidinic Endonuclease 1 Activity Detection with Ultra-High Sensitivity and Selectivity.” Sensors and Actuators, B: Chemical 330(October 2020):129332. doi: 10.1016/j.snb.2020.129332.
Janik, Monika, Ewa Brzozowska, Paula Czyszczoń, Anna Celebańska, Marcin Koba, Andrzej Gamian, Wojtek J. Bock, and Mateusz Śmietana. 2021. “Optical Fiber Aptasensor for Label-Free Bacteria Detection in Small Volumes.” Sensors and Actuators, B: Chemical 330(November 2020). doi: 10.1016/j.snb.2020.129316.
João, Jorge, João Lampreia, Duarte Miguel F. Prazeres, and Ana M. Azevedo. 2021. “Manufacturing of Bacteriophages for Therapeutic Applications.” Biotechnology Advances 49(November 2020). doi: 10.1016/j.biotechadv.2021.107758.
Kim, Hye Jin, You Tae Kim, Hyeun Bum Kim, Sang Ho Choi, and Ju Hoon Lee. 2021. “Characterization of Bacteriophage VVP001 and Its Application for the Inhibition of Vibrio Vulnificus Causing Seafood-Borne Diseases.” Food Microbiology 94(March 2020):103630. doi: 10.1016/j.fm.2020.103630.
Kotsiri, Zoi, Jasmina Vidic, and Apostolos Vantarakis. 2022. “Applications of Biosensors for Bacteria and Virus Detection in Food and Water–A Systematic Review.” Journal of Environmental Sciences (China) 111:367–79. doi: 10.1016/j.jes.2021.04.009.
Li, Junjie, Yameng Li, Yifeng Ding, Chenxi Huang, Yu Zhang, Jia Wang, and Xiaohong Wang. 2021. “Characterization of a Novel Siphoviridae Salmonella Bacteriophage T156 and Its Microencapsulation Application in Food Matrix.” Food Research International 140(November 2020):110004. doi: 10.1016/j.foodres.2020.110004.
Muntean, Cristina M., Nicoleta E. Dina, Ioan Bratu, Carmen Tripon, Sorina Niţu (Năstase), and Ana Coste. 2021. “Acidic PH-Responsive Changes of DNA Structure and Surface Dynamics as Probed with Ultrasensitive Raman Spectroscopy.” Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy 258. doi: 10.1016/j.saa.2021.119866.
Pellizza, Leonardo, José L. López, Susana Vázquez, Gabriela Sycz, Beatriz G. Guimarães, Jimena Rinaldi, Fernando A. Goldbaum, Martín Aran, Walter P. Mac Cormack, and Sebastián Klinke. 2020. “Structure of the Putative Long Tail Fiber Receptor-Binding Tip of a Novel Temperate Bacteriophage from the Antarctic Bacterium Bizionia Argentinensis JUB59.” Journal of Structural Biology 212(1):107595. doi: 10.1016/j.jsb.2020.107595.
Shi, Yanli, Yong He, Lvxia Zhang, Lin Wang, and Zhifeng Fu. 2021. “Dual-Site Recognition of Pseudomonas Aeruginosa Using Polymyxin B and Bacteriophage Tail Fiber Protein.” Analytica Chimica Acta 1180:338855. doi: 10.1016/j.aca.2021.338855.
Zhao, Shanshan, Yuansheng Wu, and Jianguo Wu. 2021. “Arms Race between Rice and Viruses: A Review of Viral and Host Factors.” Current Opinion in Virology 47:38–44. doi: 10.1016/j.coviro.2021.01.002.
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Copyright (c) 2023 Ferry Irawan, Dharma Gyta Sari Harahap, Muh. Rafi'y (Author)
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