Dampak Media Subkultur dan Variasi Subkultur untuk Pertumbuhan Tunas pada Kultur Jaringan Pisang (Musa spp.) Serta Penerapan Penanaman Bibit Dengan Pemberian Agen Hayati
Keywords:
Adaptation, Innovative Industry, In Vitro, Subculture, Sustainable AgricultureAbstract
This study aims to evaluate the impact of subculture media on shoot proliferation in banana (Musa spp.) tissue culture. The experiment was conducted using Murashige & Skoog (MS) medium and a modified medium, with subculture variations of 2, 4, and 6 cycles. The observed parameters included the number of shoots, shoot height, root count, and root length. Results showed that shoot and root growth in the modified medium with activated charcoal and four subculture cycles produced the best shoot proliferation response compared to other treatments. The combination of culture media and subculture frequency significantly enhances propagation efficiency in sustainable elite seedling production. This strategy offers an innovative solution for modern agriculture, addressing efficiency challenges amid production instability, climate change threats, and land degradation.
Downloads
References
Anderson, P., & Clarke, J. (2021). Tissue culture adaptation for sustainable agriculture. Plant Science Today, 17(2), 102-118.
Bidhari, L.A. (2018). Pengaruh Jenis Media dan Konsentrasi Ekstra Buah Tomat terhadap Multiplikasi Tunas Pisang Ambon Secara In Vitro. Tesis. UNS Surakarta
Chen, Y., & Zhang, T. (2021). Effects of plant growth regulators on banana shoot multiplication. Journal of Horticultural Science, 14(3), 178-190.
Elfiani & Jakoni. (2015). Sterilisasi eksplan dan sub kultur anggrek, sirih merah dan krisan pada perbanyakan tanaman secara in vitro. Jurnal Dinamika Pertanian. XXX(2), 117– 124.
Hardjono, T., & Wibowo, A. (2021). Teknik kultur jaringan dalam perbanyakan pisang unggul di Indonesia. Jurnal Hortikultura Tropis, 12(3), 55-67.
Hutami, S. 2008. Ulasan Masalah Pencoklatan pada Kultur Jaringan. Jurnal AgroBiogen. 4(2): 83-88.
Idly, Saputra, N., Lusmaniar & Syamsudin, T. (2021). Pengaruh Penambahan Ekstra Nabati ke dalam Media Alternatif Subkultur Terhadap Planlet Anggrek Dendrobium sp. [Thesis]. Palembang: Universitas Tamansiswa Palembang.
Maulana, M., Zuhra, F., Pratiwi, H., & Haharap, D. E. Application of Mycorrhizal Fungi and Application of Organic Matter in Coastal Sandy Soil Media with Copy Grip on the Growth of Cayenne Pepper.( Pakistan Journal of Life and Social Sciences. 22(2): 20182-20190)
Maulana, M., Pratiwi, V., Harta, R. Y., Ritaqwin, Z., & Harahap, D. E. (2024). Produksi Tanaman Okra (Abelmoschus esculentus L. Moench) Akibat Aplikasi Mikoriza dan Pupuk Rock Phosphat pada Cekaman Salin. Jurnal Ilmu Pertanian Indonesia, 29(4), 533-540.
Maulana, M., Safriani, S. R., & Ritaqwin, Z. (2024). Morfologi Koloni Bakteri Endofit dari Akar Tanaman Kopi Arabika. Science Tech: Jurnal Ilmu Pengetahuan dan Teknologi, 10(2), 90-95.
Maulana, M., & Harahap, D. E. (2023). Peningkatan Produksi Tanaman Okra Akibat Pemberian Beberapa Jenis Mikoriza Dan Dosis Rock Phosphat Pada Tanah Salin. Journal Agro-Livestock (JAL), 1(01), 14-26.
Maulana, M., Ritaqwin, Z., & Mawaddah, F. (2022). Pertumbuhan dan kolonisasi fungi mikroriza terhadap cekaman tanah salin pada tanaman cabai. Viabel: Jurnal Ilmiah Ilmu-Ilmu Pertanian, 16(1), 1-12.
Maulana, M. (2020). Pertumbuhan beberapa varietas cabai (Capsicum annum L.) akibat Applikasi Mikoriza Pada Tanah Salin. Fanik: Jurnal Faperta Uniki, 1(1), 9-16.
Ramesh, Y., dan V. Ramassamy. 2014. Effect of geling agents in in vitro multiplication of banana var. Poovan. Int, J. Advanced Bio. Research. 4(3): 308-311.
Rodinah R, Hardarani N, & Ariani HD. (2018). Modifikasi media dan periode subkultur pada kultur jaringan pisang talas (Musa parasidiaca VAR. SAPIENTUM L.). Jurnal Hexagro, 2(2), 1-6.
Smith, J., & Brown, L. (2023). Tissue culture techniques for sustainable agriculture. Agricultural Biotechnology Journal, 30(5), 120-138.
White, P., & Green, R. (2024). Micropropagation strategies for tropical fruit crops. Horticultural Science Review, 22(3), 67-89.
Widodo, H., & Setiawan, I. (2023). Efektivitas metode sterilisasi dalam kultur jaringan pisang. Jurnal Agrobioteknologi, 21(3), 178-190.
Wilson, T., & Harris, M. (2022). Challenges in banana tissue culture propagation. Biotech Reports, 16(1), 56-72.
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Era Maulia, Wika Rahmatika, Mizan Maulana (Author)
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
